Figure 5.

The washed platelets assay was performed with venous blood collected from healthy volunteers. The blood was centrifuged in 3.8% trisodium-citrate for 10 minutes at 200 × g and the residue was centrifuged again for 20 minutes at 1500 × g. Aggregations were performed in 1 μg and 3 μg concentrations in 20 μL of BTL-2, PLA₂ and the two proteins (v/v). 5a) BTL-2 induced low aggregation, however the PLA₂ induced high aggregation at these concentrations, and both proteins induced a lower aggregation when compared with the PLA₂ alone.