Figure 7.

Male Wistar rats (120 g–150 g) were anaesthetised with halothane (inhaled). The paw oedema was induced by a single subplantar injection of 100 μL of sample 100 μg/paw). Paw volume was measured immediately before the injection and the intervals thereafter (15, 30, 60 and 120 minutes) using a hydroplethysmometer (model 7150, Ugo Basile, Italy). 7a) BTL-2 induced similar oedema simihat of the saline control. PLA₂ showed the highest oedema at 30 minutes before the injection. The presence of BTL-2 decreased the oedema induced by the enzyme. 7b) The compound 48/80 (C48/80) was used, incubated with BTL-2, to show that the lectin was not acting on the tissues to decrease the action of PLA₂, but was probably binding to the enzyme. Thus, the lectin did not decrease the action of C48/80 act.