Table 1.
Membrane modifications after hormone treatments in different breast cancer cell lines
| Treatment (15 min) | Mean membrane thickness (pixel ± SD)(× 1000) | Mean membrane intensity (mean gray level ± SD) | Mean cytosol intensity (mean gray level ± SD) | % cells with actin modifications | |
| T47-D | CON | 32.4 ± 5.8 | 61.5 ± 8.3 | 59.8 ± 5.6 | 5.2 ± 0.8 % |
| P | 62.3 ± 9.2* | 102.2 ± 10.4* | 62.3 ± 7.4 | 64.5 ± 5.4 % * | |
| P+E2 | 78.2 ± 10.3* | 124.5 ± 13.2* | 58.5 ± 6.1 | 72.2 ± 5.7 % * | |
| MPA | 76.3 ± 14.6* | 118.6 ± 12.7* | 67.8 ± 8.2 | 69.6 ± 6.1 % * | |
| MPA+E2 | 92.5 ± 12.3* | 131.8 ± 12.4* | 57.4 ± 6.6 | 81.8 ± 4.9 % * | |
| DRSP | 54.6 ± 6.7 * | 87.2 ± 8.3 * | 61.6 ± 8.2 | 60.4 ± 4.4 % * | |
| DRSP+E2 | 60.3 ± 5.2 * | 98.5 ± 11.6* | 57.8 ± 6.7 | 68.1 ± 5.2 % * | |
| NES | 71.5 ± 8.6* | 110.2 ± 14.4 * | 61.4 ± 8.5 | 69.6 ± 6.5 % * | |
| NES+E2 | 80.6 ± 10.5 * | 127.3 ± 7.2 * | 59.1 ± 5.4 | 73.1 ± 5.6 % * | |
| MCF-7 | CON | 46.6 ± 4.7 | 77.8 ± 5.8 | 74.3 ± 6.2 | 6.7 ± 1.3 % |
| P | 99.2 ± 7.4 * | 132.4 ± 8.6 * | 68.5 ± 4.7 | 70.6 ± 7.4 % * | |
| P+E2 | 115.8 ± 8.3 * | 140.3 ± 10.2 * | 66.3 ± 5.2 | 68.3 ± 6.2 % * | |
| MPA | 108.4 ± 11.3 * | 142.7 ± 12.5 * | 60.4 ± 5.6 | 78.2 ± 7.8 % * | |
| MPA+E2 | 123.6 ± 10.8 * | 150.2 ± 14.6 * | 62.5 ± 4.9 | 80.5 ± 7.2 % * | |
| MDA-MB-468 | CON | 31.2 ± 4.4 | 66.8 ± 7.2 | 62.6 ± 5.5 | 5.4 ± 0.7 % |
| P | 34.3 ± 4.1 | 70.5 ± 6.4 | 64.2 ± 5.8 | 4.8 ± 0.6 % | |
| P+E2 | 32.5 ± 3.9 | 68.1 ± 5.8 | 61.4 ± 4.3 | 5.1 ± 0.4 % | |
| MPA | 36.2 ± 5.2 | 67.2 ± 5.5 | 70.5 ± 5.3 | 4.4 ± 0.8 % | |
| MPA+E2 | 32.2 ± 4.6 | 63.6 ± 6.2 | 66.3 ± 5.6 | 5.2 ± 0.7 % | |
| MDA-MB-468 (transfected with PR) | CON | 34.6 ± 4.3 | 65.4 ± 6.8 | 70.4 ± 5.2 | 5.2 ± 0.9 % |
| P | 66.7 ± 6.8 * | 97.4 ± 8.3 * | 66.3 ± 4.8 | 58.6 ± 6.7 % * | |
| P+E2 | 72.5 ± 7.1 * | 106.3 ± 10.6 * | 72.5 ± 6.4 | 60.3 ± 6.9 % * | |
| MPA | 70.4 ± 7.4 * | 102.8 ± 11.8 * | 68.2 ± 5.5 | 60.2 ± 6.6 % * | |
| MPA+E2 | 76.2 ± 8.2 * | 118.5 ± 13.2 * | 71.8 ± 6.8 | 65.8 ± 7.1 % * |
The table displays the mean thickness of the cell membrane, the mean actin intensity of the membrane and of the cytoplasm, as well as the % cells with spatial actin modifications in several breast cancer cell lines treated with different regimens of hormones (P, MPA, DRSP, all 100 nM; NES, 1 nM; E2, 10 nM) for 15 min. Analytic results were obtained by using Leica QWin image analysis and processing software.