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. 2008 Jun 24;9:60. doi: 10.1186/1471-2199-9-60

Figure 4.

Figure 4

mRNAs deregulated by GFP siRNA contain short sequences perfectly matched to the sense or antisense strand of GFP siRNA. In order to shed light on the molecular mechanism of sequence-specific off-target effects, we analyzed the mRNAs modulated after transfection of GFP siRNA for sequence homology to the transfected siRNA molecules. The number of perfect matches with a homology length of at least 8 bp was significantly higher using the proper GFP siRNA sequences as compared to the shuffled siRNA sequences.