Fig. 1.

The structure of mFu gene and generated cDNA clones. a The mFu gene found on Chromosome 1 consists of 28 exons. The translated exons are 3–28 encoding an N-terminal serine/threonine kinase domain (exons 3–9; dark gray) and a C-terminal region (exons 10–28; light gray) suggested having regulatory functions. Untranslated parts are shown in black. b Schematic representation of the FuFL and Fu(Δ6–26) cDNA clones obtained by RT-PCR as shown (arrows) in (c). Compared to the FuFL cDNA the Fu(Δ6–26) lacks a big part from exon 6 to 26 indicated by dashed lines. The different RPA hybridizing probes are also indicated in (b). Probe 1 consist of 323 nt before and 76 nt after alternative splicing in Fu(Δ6–26) giving a 399 nt long protected fragment. Probe 2 was complimentary to 449 nt of exon 27 present in both FuFL and Fu(Δ6–26). Probe 3 covers 550 nt from the end of exon 4 to the middle of exon 7 and is FuFL specific