Fig. 1.

Wnts 3a and 10b induce gene expression in fibroblasts. Wnt3a induces mRNA expression in fibroblasts. NIH 3T3 fibroblasts were treated with or without Wnt3a (150 ng/ml, 6 h). RNAs were harvested, and subjected to real time PCR analysis to detect 18S rRNA and RNAs as indicated. Experiments were performed thrice. A representative experiment is shown (three replicates). Variation within samples was less than 10%. NIH 3T3 fibroblasts were also treated with or without conditioned media prepared from cells stably transfected with empty expression vector or vector encoding Wnt10b. RNAs were harvested, and subjected to real time PCR analysis to detect 18S rRNA and RNAs as indicated. Untreated or vector-alone-treated samples are expressed as 1 (control). Experiments were performed at least twice. A representative experiment is shown (three replicates). CCN2, TSP-1 (thrombospondin-1), VEGF-C, PLOD2, TGFβ2 and ET-1 transcripts were examined. Variation within samples was less than 10%. Significance is indicated by an asterisk