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. 1999 Dec 7;96(25):14312–14317. doi: 10.1073/pnas.96.25.14312

Figure 2.

Figure 2

(Left) Freeze fracture electron micrographs of a 45 mM DMPG dispersion. (A) At 9°C, displaying large vesicles (representative features of different fields are given). The membrane sheets are overlaid by short periodic ripples. (B) At 24.5°C, displaying membrane sheets, arranged randomly toward the fractured surface and continuous membrane segments (representative features of different fields are given). The membrane sheets are overlaid by large periodic ripples. In some segments these ripples can be seen in a cross section. (C) At 50°C, displaying perfectly spherical vesicles of various sizes (representative features of different fields are given). The vesicles on average are significantly smaller than in A. (Right) Cryo-TEM micrographs of a 15 mM DMPG dispersion. (A) At 15°C, displaying large unilamellar vesicles (representative features of different fields are given) that are not round in shape. (B) At 25°C, displaying randomly curved (Right) or regularly spaced (Left) continuous membranes (representative features of different fields are given). (C) At 45°C, displaying unilamellar vesicles of various sizes (representative features of different fields are given). Some vesicles are situated within larger vesicles.