Figure 2.

Picosecond time-resolved mid-IR absorbance spectra of photolyzed Hb¹³CO. Photolysis of Hb¹³CO detaches ¹³CO from the heme. The ¹³CO is produced predominately in its ground vibrational state (ν = 0) and gives rise to two major features, B₁ and B₂. A small (3.6%) portion of the ¹³CO is produced in its excited vibrational state (ν = 1) and gives rise to two satellite features, B^*₁ and B^*₂. Vibrational relaxation back to the ground state (ν = 1 → 0) causes the red-shifted satellite features to disappear. The solid curves were obtained by a least squares fitting to experimental spectra with the constrained model involving the Gaussian functions.