Figure 3.

Dual immunofluorescent staining for somatostatin receptors and smooth muscle α-actin (SMA) in glomeruli. Staining patterns of somatostatin receptors in the glomeruli (green, FITC) and double stained by anti-smooth muscle α-actin (red, Cy3). Merged pictures with nuclear staining (blue, 4′-6-diamidino-2-phenylindole) are the glomeruli with 6× zoom for left photo images. No staining for normal rabbit serum control (Control). There was no overlapping staining for von Willebrand factor (VWF) and SMA (VWF + SMA). Overlapping staining (yellow) is indicated by arrowheads in merged images. Bar = 50 μm.