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. 2008 Aug;56(8):733–743. doi: 10.1369/jhc.2008.950998

Figure 5.

Figure 5

Detection of somatostatin receptor mRNA in kidney by RT-PCR. (A) PCR products were shown in the 2% agarose gel stained with ethidium bromide. The expected size of PCR amplicons is 68 bp for sst1, 61 bp for sst2, 86 bp for sst3, 68 bp for sst4, and 79 bp for sst5. (B) The spliced variants of sst2A [Lane 1, 332 bp with reverse primer (1); Lane 2, 475 bp with reverse primer (2)] and sst2B (95 bp) were detected by RT-PCR. The housekeeping gene, GAPDH (326 bp), was used as control. The sst2B product was confirmed by direct sequencing. (C) An example of real-time PCR detection of somatostatin mRNA in kidney. No product was detected if the nonspecific probes for somatostatin were used, such as sst1 probe used for detection of sst2 (data not shown).