FIGURE 1.

A2^–/– tissues express low levels of p11 protein. A, total RNA (1 μg) isolated from lung, heart, liver, kidney, and spleen tissue from A2^+/+ and A2^–/– mice was subjected to reverse transcription-PCR analysis using gene-specific mouse A2, p11, or GAPDH primers. B, protein extracts (40 μg) from lung, heart, liver, kidney, and spleen from A2^+/+ and A2^–/– mice were resolved on 15% SDS-polyacrylamide gels and immunoblotted with IgG directed against A2, p11, or GAPDH. C, A2-myc- and GFP-encoding expression plasmids were cotransfected into mouse A2^–/– CMEC by electroporation. GFP-positive cells were selected by fluorescence-activated cell sorter, and lysates were immunoblotted for A2, p11, and β-tubulin (β-tub).