FIGURE 5.

Identification of the p53 protein-binding sequences on PAI-1 3′-UTR mRNA. A, deletion map indicating the p53 protein-binding site on PAI-1 mRNA. B, rp53 protein was incubated with ³²P-labeled PAI-1 mRNA full-length coding region (1) or full-length 3′-untranslated region (2) or 3′-UTR deletion transcripts (3′-UTR-DL-3–11). The RNA·protein complex was analyzed by gel mobility shift assay. Arrow indicates the PAI-1 mRNA·p53 protein complex. Fp, ³²P-labeled PAI-1 mRNA probe is incubated with buffer alone. Data are representative of four independent analyses.