Retinyl esters in wild-type and
rpe65-/-
mice. A, levels of all-trans-REs in light-adapted
(LA) and dark-adapted (DA) eyes from wild-type mice.
Expressed as picomoles per eye, ± S.D. (n = 4).
Asterisk denotes a significant difference between DA and LA values
(p = 0.046). B, representative chromatogram of retinoids
extracted from dark-adapted, wild-type mouse eyecups in milliabsorbance units
(mAU) at 325 nm. Identified peaks are as follows: peak 1,
11-cis-retinyl palmitate; peak 2, all-trans-retinyl
palmitate; peak 3, syn-11-cis-RAL-oxime; peak
4, syn-all-trans-RAL-oxime; peak 5,
11-cis-ROL; peak 6, anti-11-cis-RAL-oxime;
peak 7, all-trans-ROL; peak 8,
anti-all-trans-RAL-oxime. C, levels of
all-trans-REs in LA and DA eyes from
rpe65-/- mice.
Expressed as picomoles per eye, ± S.D. (n = 6).
Asterisk denotes a significant difference between DA and LA values
(p = 0.038). D, representative chromatogram of retinoids
extracted from dark-adapted,
rpe65-/- mouse
eyecups in milliabsorbance units (mAU) at 325 nm. Identified peaks:
peak 2, all-trans-retinyl palmitate; peak 7,
all-trans-ROL.