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. 2008 Jul 11;283(28):19730–19738. doi: 10.1074/jbc.M801288200

FIGURE 2.

FIGURE 2.

Retinyl esters in wild-type and rpe65-/- mice. A, levels of all-trans-REs in light-adapted (LA) and dark-adapted (DA) eyes from wild-type mice. Expressed as picomoles per eye, ± S.D. (n = 4). Asterisk denotes a significant difference between DA and LA values (p = 0.046). B, representative chromatogram of retinoids extracted from dark-adapted, wild-type mouse eyecups in milliabsorbance units (mAU) at 325 nm. Identified peaks are as follows: peak 1, 11-cis-retinyl palmitate; peak 2, all-trans-retinyl palmitate; peak 3, syn-11-cis-RAL-oxime; peak 4, syn-all-trans-RAL-oxime; peak 5, 11-cis-ROL; peak 6, anti-11-cis-RAL-oxime; peak 7, all-trans-ROL; peak 8, anti-all-trans-RAL-oxime. C, levels of all-trans-REs in LA and DA eyes from rpe65-/- mice. Expressed as picomoles per eye, ± S.D. (n = 6). Asterisk denotes a significant difference between DA and LA values (p = 0.038). D, representative chromatogram of retinoids extracted from dark-adapted, rpe65-/- mouse eyecups in milliabsorbance units (mAU) at 325 nm. Identified peaks: peak 2, all-trans-retinyl palmitate; peak 7, all-trans-ROL.