TABLE 1.
DEF site motifs for MAPKs Residues positively selected at each position within the DEF site library are shown for each MAPK. Selectivity values in parentheses were calculated as described in the legend to Fig. 3. Residues with selectivity values greater than 1.5 are shown, and positions where no residues were selected to such an extent are indicated with an X. Residues with values greater than 3.0 are shown in boldface.
|
Kinase
|
Position
|
|||||||
|---|---|---|---|---|---|---|---|---|
| P1 | P2 | P3 | P4 | |||||
| ERK2 | Phe | (7.6) | Trp | (1.7) | Phe | (4.7) | Pro | (2.4) |
| Trp | (5.5) | Glu | (1.6) | Trp | (4.5) | Phe | (2.2) | |
| Val | (1.5) | Tyr | (4.3) | Trp | (1.7) | |||
| Glu | (1.6) | |||||||
| p38α | Trp | (7.5) | Glu | (2.1) | Trp | (8.8) | Pro | (2.8) |
| Phe | (3.5) | Val | (1.6) | Tyr | (2.6) | Trp | (1.8) | |
| Phe | (2.1) | Phe | (1.6) | |||||
| p38β | Trp | (4.0) | Ile | (1.8) | Trp | (5.3) | Phe | (2.3) |
| Phe | (2.7) | Glu | (1.8) | Phe | (2.8) | Trp | (1.8) | |
| Leu | (2.0) | Val | (1.8) | Tyr | (2.2) | |||
| Phe | (1.6) | |||||||
| p38γ | Leu | (2.5) | Leu | (1.7) | Trp | (3.5) | X | |
| Ile | (1.8) | Phe | (1.9) | |||||
| Pro | (1.7) | |||||||
| p38δ | Ile | (4.0) | Val | (1.7) | Trp | (3.7) | Pro | (1.9) |
| Leu | (3.7) | Phe | (2.6) | Trp | (1.8) | |||
| Pro | (2.8) | Leu | (1.6) | Phe | (1.7) | |||
| Val | (2.1) | |||||||