FIGURE 2.

Binding interaction of NADP⁺ and S-NADP⁺ with NOSr proteins. A and B, given difference spectra were calculated by subtracting the spectrum of each fully oxidized protein from the spectra of the NADP⁺ or S-NADP⁺-saturated protein. C and D, kinetics of dissociation of NADP⁺ or S-NADP⁺ from NOSr proteins. The NADP⁺- or S-NADP⁺-saturated NOSr proteins were rapidly mixed with an excess of 2′,5′-ADP in the stopped-flow instrument at 10 °C. Data are representative of at least two experiments.