FIGURE 8.

Reaction of 1-electron reduced NOSr proteins with excess cytochrome c. 1-Electron reduced CaM-free or CaM-bound NOSr proteins (5–20 μm) were rapidly mixed with a solution containing excess cytochrome c (100 μm) and NADPH (200 μm) in a stopped-flow instrument at 10 °C. Kinetic traces were recorded at 550 nm during the first few electron transfers to cytochrome c. Arrows indicate the estimated delay time before the start of linear phase cytochrome c reduction. Data are representative of at least two experiments.