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. 2008 Jul 11;283(28):19499–19510. doi: 10.1074/jbc.M800331200

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Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — The apoptosis-generated N-terminal fragment of HDAC7 is unstable. HEK293 cells transfected with FLAG-HDAC7, HDAC7-FLAG, or FLAG-HDAC7-FLAG were treated with 200 ng/ml TRAIL for the indicated times. Cell lysates were normalized for protein concentration, resolved by SDS-PAGE, and immunoblotted (IB) with the indicated antibody. Asterisks represent nonspecific bands and serve as loading controls. Although the polyclonal HDAC7 antibody is specific and recognizes the N terminus (Santa Cruz Biotechnology, N-18) the position of the epitope(s) is unknown.