Figure 4. CaMKII inhibitors inhibit myeloid leukemia cell proliferation.

A). K562 cells were seeded in liquid suspension culture at 5 × 10⁴ cells/ml in the presence or absence of the indicated concentration of compounds, and cell counts were obtained at the indicated time.

B). The indicated myeloid leukemia cell lines were seeded in liquid suspension culture at 5 × 10⁴ cells/ml in the presence or absence of KN93, and cell counts obtained at the indicated time.

C). K562 cells were electroporated with the LXSN expression vector harboring the kinase dead, Lys⁴³ mutated, truncated CaMKII construct (kdCaMKII) as well as with the control (empty) LXSN vector. The electroporated cells were diluted into 96 well plates in liquid suspension culture in the presence of G418 (1 mg/ml). Following eight days of culture the total number of discrete, actively proliferating G418 resistant colonies within individual wells was determined.