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. Author manuscript; available in PMC: 2008 Jul 7.
Published in final edited form as: Free Radic Biol Med. 2006 Jul 21;41(8):1338–1350. doi: 10.1016/j.freeradbiomed.2006.07.015

Figure 3. The rate of accumulation of H2O2 in the media increases with the cellular MnSOD activity.

Figure 3

(♦) The H2O2 released from MCF-7 cells and several clones overexpressing MnSOD (1.1-, 3-, 6-, and 19-fold) in exponential growth was determined at several time points over a period of two hours. The concentrations of H2O2 in the medium were measured using Amplex red. The ordinate represents the change in the [H2O2] in the media versus time compared to wildtype MCF-7 cells (1 on the left ordinate scale represents 2.8 zmol cell−1 s−1). Results are from three independent experiments ± standard deviation.

( ) The mitochondrial steady-state level of H2O2 (nM) from the kinetic model achieved with different levels of MnSOD (0.7–10 μM) as presented in Figure 4D.