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. Author manuscript; available in PMC: 2008 Jul 7.
Published in final edited form as: Am J Physiol Gastrointest Liver Physiol. 2003 Jun 11;285(5):G967–G979. doi: 10.1152/ajpgi.00532.2002

Figure 6.

Figure 6

Effects of enhanced restitution on recovery of barrier function after deoxycholate injury to porcine ileal mucosa mounted in Ussing chambers. Restitution was stimulated by addition of L-arginine (ARG; 5 mM) and fetal bovine serum (FBS; 1%) to the serosal and mucosal reservoir immediately after injury and prior to onset of repair. Results are shown for mucosae at peak repair (A, B). Indomethacin (INDO; 5×10−6M) did not attenuate baseline or stimulated restitution despite arrest of villus contraction during recovery. Magnification, 250X. Barrier function, as determined by measures of transepithelial electrical resistance (TER)(C) and mucosal to serosal flux of 3H-mannitol (Jm→s) (D), was abolished in the absence of endogenous prostaglandin (PG) synthesis, despite nearly complete restitution of villi. (✱p<0.05, ✱✱p<0.01, ✱✱✱p<0.001; one-way ANOVA). Values represent mean ± SE; n = number of pigs. Bar = bile salt exposure.