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. 2008 Jul 7;105(28):9633–9638. doi: 10.1073/pnas.0803749105

Fig. 1.

Fig. 1.

Lack of inhibition of HDAC6 proteins induces accumulation of aetylated 50- to 22-kDa proteins. (A) Western blot analysis of lysates of prostate cancer cells: lane 1, LAPC4; lane 2, Du145; lane 3, LNCaP; lane 4, PC3. α-HDAC6, HDAC6; α-Ac-Tub, acetylated α-tubulin; α-Tub, α-tubulin as loading control. (B) Western blot analysis of lysates of prostate cell lines as in A were cultured without (−) or with (+) 5 μM SAHA for 24 h. (C) Western blot analysis of LNCaP cells cultured with tubacin, SAHA, or MS-275 (5 μM each) for the times indicated. (D) Western blot analysis of LAPC4 cells transfected with HDAC6 gene (Left) or LNCaP cells transfected with HDAC6 siRNA oligonucleotides (Right).