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. 2008 Jul 7;105(28):9633–9638. doi: 10.1073/pnas.0803749105

Fig. 2.

Fig. 2.

Identification of acetylated peroxiredoxins. (A) Detection of Prx I and Prx II in LAPC4 immunoprecipitate (IP) generated with anti-acetylated tubulin (α-Ac-tub) antibody. (B) Flagged Prx I and Prx II overexpressed in LAPC4 cells were immunoprecipitated with FLAG antibody and analyzed by Western blotting (WB) with the anti-acetylated lysine antibody (α-Ac-Lys). (C) Prx I and Prx II recombinant proteins were acetylated in vitro, fractionated in SDS gel, and probed with anti-acetylated lysine antibody. (D) Recombinant Prx I wild type (WT) and mutants (K197Q and K197R) were acetylated in vitro. (E) Flagged Prx II proteins overexpressed in LAPC4 cells were immunoprecipitated and probed with anti-acetylated lysine antibody.