Figure 1.
EP2 and EP4 expression in glioblastoma cells are increased relative to low grade glioma cells. 2 low grade glioma cell lines (SW1783 and SW1088) and 5 glioblastoma cell lines (A172, T98G, U87MG, U138MG and U373MG) were grown to 80–90% confluency in the appropriate media. Total protein (50 µg) was subjected to Western blot analysis. The anti-Actin antibody was used for a loading control. STD represents PPARγ standard that was made by transfection of 5 µg PPARγ wild type expression plasmid into T98G cells.