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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2008 Feb 20;1783(6):1211–1219. doi: 10.1016/j.bbamcr.2008.01.032

Figure 8.

Figure 8

Sp-1 is essential for the expression of EP4 in T98G cells. The pEP4-3 or pGL3 and Sp-1 expression plasmids or pcDNA3.1 were co-transfected into T98G cells for 24h. After 24h treatment with TGZ or Control, luciferase activity was measured (A). *; P<0.01, significant compared with pcDNA3.1+Control; †; P<0.005, significant compared with Sp-1+TGZ. Sp-1 or Control siRNA was transfected into T98G cells and the knock-down of Sp-1 was confirmed by Western blot analysis (B). After transfection of Sp-1 or Control siRNA, EP4 protein was measured with Western blot analysis (C).