Skip to main content
letter
. 2008 May 27;52(7):2693–2695. doi: 10.1128/AAC.01655-07

TABLE 1.

Deduced amino acid substitutions in PBP2B, PBP2X, and PBP1A of 25 pneumococcal strainsa

Strain Resistance phenotype Serotype MICb Amino acid substitutions in PBP2B at position:
No. of amino acid substitutions in PBP2B:
Amino acid substitutions in PBP2X at position:
No. of amino acid substitutions in PBP2X:
Amino acid substitutions in PBP1A at position:
No. of amino acid substitutions in PBP1A:
Total amino acid substitutionsc
443 451 481 485 488 494 614 624 629 630 633 635 DM TP ORF 338 339 343 346 347 364 369 371 384 394 400 546 550 552 595 605 DM TP PASTA ORF 371 388 432 546 574 575 576 577 606 609 611 612 TG TP ORF
R6 S 0.0125 Q T E S G T L A A D Q T T M M A A L A I R H M L T Q Y N T E P N T S Q F L N L T
ATCC BAA-334 S 4 0.0125 . . . . . . . . . . . . 0 0 0 . . . . . . . . . . . . . . . . 0 1 0 1 . D . . . . . . . . . . 1 2 8 9
URA4929 S 9V 0.0125 . . . . . . . . . . . . 0 0 0 . . . . . . . . . . . . . . . . 0 2 0 2 . D . . . . . . . . . . 1 2 7 9
URA4376 T, E, C 6B 0.0125 . . . . . . . . . . . . 1 0 1 . . . . . . . . . . . . . . . . 0 1 0 1 . D . . . . . . . . . . 1 2 8 10
URA3537 S 3 0.0125 . . . . . . . . . . . . 1 1 2 . . . . . . . . . . . . . . . . 0 1 0 2 . D . . . . . . . . . . 1 2 7 11
URA2543 S NT 0.025 . . . . . . . . . . . . 2 0 2 . . . . . . . . . . . . . . . . 0 1 0 1 . D . . . . . . . . . . 4 1 10 13
URA4933 E 14 0.0125 . . . . . . . . . . . . 3 0 3 . . T . . . . . . . . . . . . . 0 1 0 2 . D . . . . . . . . . . 1 1 9 14
URA3558 P, T, E, C 23 0.2 . . . . . . . . . . . . 3 1 4 . . T . . . V . . L . . . . . . 16 12 3 37 . D . . . . . . . . . . 1 4 56 97
URA4087 S 19F 0.025 . . . . . . . . . . . . 0 0 0 . . . . . . . . G . . . . E . . 0 13 3 26 . D . . . . . . . . . . 2 3 11 37
URA4566 P, T, E, C 6B 0.1 . A G . . S . . . G E N 0 13 18 A . T . . . V . G . . . . . . . 3 8 1 17 . D . . . . . . . . . . 1 1 7 42
URA2542 S 22 0.05 . A G . . S S . E G E N 1 12 17 . . T . . . . . G . . . . E . . 0 10 0 10 . D . . . . . . . . . . 1 1 7 34
URA3635 P 19A 0.1 . A G . . S . . . . . . 6 6 13 A . T . S . V . G . . . . . . . 1 9 4 19 . D . . . . . . . . . . 1 2 8 40
URA4731 P 14 0.1 E A G . A A A . E G E N 1 32 37 . . . . . . . . G . . . . E . . 2 24 5 44 . D . . N T G Y . D . . 1 11 19 100
URA5391 P 23F 0.1 E A G . A A S . E G E N 1 34 40 . . T . . . . . G . . . . E . . 4 23 15 54 . D . . . . . . . . . . 2 2 9 103
URA5779 P, T, E, C 15A 0.1 . A G . . S A . E G E N 1 10 16 . . . . . . . . . . . . . E . . 1 21 2 27 . D . . . . . . . . . . 3 28 43 86
ATCC 700670 P, X, R, T, F 6B 2 E A G . . S A . . G E N 2 11 14 A . T S S F . T G . . V . . . T 2 37 1 45 A D T G N T G Y I D F L 2 44 60 119
ATCC700673 P, T, E, C 19A 2 . A G . . S . . . . . . 2 15 18 A . T S S S V T G . . . . . . . 3 28 20 65 . D . G N T G Y V D . . 13 44 90 173
URA5805 P, X, R, T, E, C 19A 1.6 E A G A . A T G . G E N 9 31 50 A . T S S F . T G . . V . . . T 3 34 18 70 S D T G N T G Y I D F L 12 43 80 200
ATCC 51916 P, X, R, E 23F 0.1 . . . . . . . G . G E N 2 3 14 A F . S S F . T G . T V A . . T 2 37 21 74 S D T G N T G Y I D F L 1 47 60 148
URA3543 P, X, R 14 2 E A G A . A T G . G E N 5 21 37 A . T S S F . T G . . V . . . T 8 35 21 79 A D T G N T G Y I D F L 2 44 52 168
URA6056 P, X, R ND 3.2 E A G . . S T G . G E N 6 33 50 A F . S S F V T G . T V . . F T 3 42 23 82 A D T G N T G Y I D F L 2 37 52 184
URA3043 P, X, R 14 2 E A G A . A . . . . . . 7 12 20 A . T S S F . T G . . V . . . T 2 37 21 73 A D T G N T G Y I D F L 2 47 55 148
URA4203 P, X, R, T, E, C 14 1.6 E A G A . A . . . . . . 7 12 20 A . T S S F . T G . . V . . . T 3 37 21 75 A D T G N T G Y I D F L 2 36 43 138
URA5128 P 14 0.5 E A G A . A . . . . . . 7 12 20 A . T S S F . T G . . V . . . T 3 37 21 75 A D T G N T G Y I D F L 2 37 52 147
URA3699 P, T, E, C 19F 0.5 E . G . A A A . . G K N 37 33 83 A . T S S F . T G . . . . . . . 18 32 20 104 A D T G N T G Y I D F Y 1 42 70 257
URA5450 P, T, E, C 24F 0.5 . A G . . S . . . . . . 4 12 17 . . . . . . . . G . . . . E . . 1 20 3 25 A D T G N T G Y I D F Y 1 42 70 112
a

The main motifs in the transpeptidase domain in PBP2B are 391SVVK, 448SSN, and 619KTG; in PBP2X are 337STMK, 395SSN, and 547KSG; and in PBP1A are 370STMK, 428SRN, and 557KTG. The amino acid positions of strain R6 were used as reference. The positions within or close to conserved motifs in the PBPs are shown. The dots indicate homologous amino acids. DM, dimerization domain; TP, transpeptidase domain; ORF, signifies amino acid substitutions encoded in the open reading frame; PASTA, penicillin-binding protein and serine/threonine kinase-associated domain; TG, transglycosylase domain; S, susceptible; T, tetracycline; E, erythromycin; C, clindamycin; F, chloramphenicol; P, penicillin; X, cefotaxime; R, ceftriaxone; ND, not determined.

b

MIC values are against penicillin (μg/ml); strains were considered penicillin susceptible for MICs ≤0.06 μg/ml, intermediate for MICs of 0.1 to 1 μg/ml, and resistant for MICs ≥1.6 μg/ml.

c

Total number of amino acid substitutions in PBP2B, PBP2X, and PBP1A.