TABLE 2.
In vitro antifungal activities of antifungal drug controlsa
| Species and strain or isolate | IC50/MIC/MFC (μM) of:b
|
|||||
|---|---|---|---|---|---|---|
| AMB | FLU | KTC | CASPO | UDA | TRB | |
| C. albicans | ||||||
| ATCC 14053 | 0.3/1.0/1.4 | 0.5/1.3/− | 0.01/0.02/− | 0.05/0.10/0.26 | >542.7c/−/− | 13.7/−/− |
| ATCC 60193 | 0.2/1.0/1.4 | 0.4/0.6/− | 0.01/0.02/− | 0.03/0.10/0.52 | >542.7/−/− | 5.3/−/− |
| ATCC 32354 | 0.3/1.0/1.4 | 0.8/5.7/− | 0.01/0.03/− | 0.07/0.13/1.03 | >542.7/−/− | 2.6/−/− |
| ATCC 90028 | 0.3/1.0/1.4 | 0.8/6.4/− | <0.01/0.01/− | 0.06/0.19/0.26 | −/−/− | 10.8/−/− |
| ATCC 200955 | 0.6/2.7/2.7 | 0.4/1.3/− | <0.01/0.01/− | 0.05/0.13/0.13 | >542.7/−/− | 3.1/51.5/− |
| Isolate 1d | 0.2/1.0/1.4 | 0.8/5.1/− | <0.01/0.10/− | 0.05/0.10/0.26 | >542.7/−/− | 11.2/−/− |
| Isolate 17d | 0.2/1.0/1.4 | 163.3/−/− | 0.3/−/− | 0.04/0.13/0.26 | >542.7/−/− | >68.3/−/− |
| Other Candida spp. | ||||||
| C. glabrata ATCC 90030 | 0.4/1.4/1.4 | 29.5/81.6/− | 0.7/4.7/- | 0.05/0.13/0.26 | −/−/− | −/−/− |
| C. krusei ATCC 6258 | 0.7/2.0/2.7 | 125.7/163.3/− | 0.4/1.1/− | 0.1/0.4/1.0 | >542.7/−/− | >68.3/−/− |
| C. parapsilosis ATCC 22019 | 0.3/2.0/5.4 | 3.4/10.2/− | 0.04/0.13/− | 0.2/0.5/− | >542.7/−/− | 0.4/42.9/− |
| C. tropicalis ATCC 750 | 0.3/1.0/1.4 | 4.3/15.3/− | 0.04/0.19/− | 0.06/0.10/0.26 | >542.7/−/− | 45.4/−/− |
| C. neoformanse | ||||||
| ATCC 90113 | 0.3/0.7/0.7 | 8.5/20.4/163.3 | 0.1/0.3/1.2 | 0.4/1.0/2.1 | 25.3/271.3/542.7 | 0.5/1.1/1.1 |
| ATCC 66031 | 0.08/0.34/0.34 | 0.7/5.1/20.4 | 0.02/0.15/0.59 | 0.1/0.5/1.0 | 2.7/135.7/271.3 | 0.4/4.3/8.6 |
| Aspergillus spp. | ||||||
| A. fumigatus ATCC 204305 | 1.2/2.0/10.8 | −/−/− | 2.7/4.7/− | ?f/−/− | −/−/− | 1.1/4.3/− |
| A. fumigatus ATCC 90906 | 0.9/1.4/2.7 | −/−/− | 3.2/7.1/− | 0.2/16.5/− | −/−/− | 1.0/4.3/− |
| A. flavus ATCC 204304 | 1.5/2.7/− | −/−/− | 2.8/4.7/− | ?f/−/− | −/−/− | 0.3/2.1/8.6 |
| A. niger ATCC 16404 | 0.9/2.0/− | −/−/− | 6.0/9.4/− | −/−/− | 414.9/−/− | 0.7/2.1/− |
| Trichophyton spp. | ||||||
| T. mentagrophytes ATCC MYA-4439 | 0.9/1.4/1.4 | 24.2/81.6/− | 0.1/0.7/− | 0.2/−/− | 378.9/542.7/− | <0.02/0.17/0.27 |
| T. mentagrophytes ATCC 9533 | 0.6/2.0/2.7 | 29.5/61.2/− | 0.2/0.4/− | 0.3/−/− | 279.0/542.7/− | <0.02/0.05/0.27 |
| T. rubrum ATCC MYA-4438 | 0.6/1.4/5.4 | 2.7/6.4/− | 0.01/0.08/− | 0.06/0.10/− | 111.3/542.7/− | 4.2/21.4/− |
| T. rubrum ATCC 10218 | 0.6/1.4/1.4 | 1.8/1.9/163.3 | 0.02/0.04/− | −/−/− | 270.0/542.7/− | <0.02/0.10/0.13 |
The highest test concentrations of AMB, FLU, KTC, CASPO, UDA, and TRB were 10.8, 163.3, 9.4, 16.5, 542.7, and 68.3 μM, respectively.
Values are means from two independent experiments, each with two replicates; the run-to-run variability was within one- or twofold. −, not active at the highest test concentration.
Some growth inhibition (25 to 49% inhibition) was found at that concentration.
Patient isolates were obtained during fluconazole therapy; isolate 1 (first isolate) and isolate 17 (last isolate) were azole resistant (35).
Values are means from one experiment with two replicates.
XLFit could not calculate an IC50, as growth was present at high and low test concentrations but inhibition was observed at mid-concentrations, possibly due to a concentration effect.