FIG. 3.
Transcript start sites and promoter regions of the blaOXA-58 gene predicted based on results of sequence analysis and 5′ RACE-PCR. Two transcripts were detected by 5′ RACE-PCR. For the long transcript, the −10 promoter is provided by tnpA of the ΔISAba3-like element, but the −35 promoter is provided by the right IR of IS1008. For the short transcript, both the −35 and −10 promoters were within the ΔISAba3-like element. Boldface-typed and asterisk-marked characters are the initiation sites of the long and short transcripts. The boxed characters are potential promoters. Three gray blocked characters are genes coding for the transposase tnpA of IS1008, ΔISAba3, and blaOXA-58, respectively.