FIG. 3.

GCP-2/CXCL6 and its derivatives GPV-50 and APF-19 cause liposome leakage by membrane disruption. (A and B) CF-loaded liposomes were incubated with the holopeptide or one of its derivatives at the indicated concentrations in buffer alone (A) or buffer containing 150 mM NaCl (B). Leakage was determined by monitoring the fluorescence emitted at 520 nm and was expressed as a percentage of the leakage induced by Triton X-100, taken as 100%. Measurements were performed at 37°C. (C) The kinetics of leakage induction by the peptides was assessed, and results are shown.