TABLE 2.
Biochemical properties and antibacterial activities of the holopeptide GCP-2/CXCL6 and its synthetic derivatives GPV-50 and APF-19a against the indicated bacteria
| Peptide | Molecular mass (kDa) | pI | No. of amino acid residues that are:
|
MBC (μM)b for:
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|
|
S. pyogenes
|
E. coli
|
|||||||||
| Hydrophobic | Hydrophilic | Positive | Negative | 50% | 90% | 50% | 90% | |||
| GCP-2 | 7.9 | 9.75 | 26 | 34 | 13 | 5 | 0.063 ± 0.01 | 0.30 ± 0.12 | 0.24 ± 0.03 | 0.46 ± 0.01 |
| GPV-50 | 5.3 | 9.75 | 18 | 20 | 7 | 2 | 0.4 ± 0.1 | 0.9 ± 0.02 | 0.8 ± 0.04 | 7.9 ± 0.2 |
| APF-19 | 2.1 | 10.2 | 6 | 10 | 5 | 1 | 5.7 ± 0.1 | 9.5 ± 0.1 | 5.6 ± 0.03 | 9.5 ± 0.05 |
a
GPV-50 corresponds to the NH2-terminal region of GCP-2/CXCL6, and APF-19 corresponds to its COOH-terminal region.
b
Values are means ± SEM.