TABLE 2.

Primers used in this study

Primer	Orientation (5′ → 3′)	Sequence (5′ → 3′)	Purpose
FKS1-453-URA3Fa	Sense	AAAGAGACCCGTACTTGGTTACATTTGGTCACCAACTTCAGAGTGCACCATACCACAGCT	S. cerevisiae mutant generation
FKS1-649-URA3Ra	Antisense	GTATTCACCTGTACACCTCATTGCAGTGGTGGACAAAATTGGTATTTCACACCGCATAGG	S. cerevisiae mutant generation
FKS1-mut647Rb	Antisense	ATTCACCTGTACACCTCATTGCAGTGGTGGACAAAATTCTAATTGNATCTCTCAAAGATA	S. cerevisiae mutant generation
FKS1-375F	Sense	GGTCGTTTTGTCAAGCGTGA	S. cerevisiae mutant generation
FKS1-707R	Antisense	GATTTCCCAACAGAGAAAATGG	S. cerevisiae mutant generation
URA3iR2	Antisense	TGCCTTTAGCGGCTTAACTG	S. cerevisiae mutant generation
1HS1F	Sense	AATGGGCTGGTGCTCAACAT	Candida universal FKS1 primers
1HS1R	Antisense	CCTTCAATTTCAGATGGAACTTGATG	Candida universal FKS1 primers
1HS2F	Sense	AAGATTGGTGCTGGTATGGG	Candida universal FKS1 primers
1HS2R	Antisense	TAATGGTGCTTGCCAATGAG	Candida universal FKS1 primers
CoinsHS1F	Sense	GGTATGGTGATATTGTCTG	C. orthopsilosis hot spot 1 sequencing
CoinsHS2R	Antisense	GGTATGGTGATATTGTCTG	C. orthopsilosis hot spot 2 sequencing
CminsHS1F	Sense	CAGAGAACATTTGTTAGCC	C. metapsilosis hot spot 1 sequencing
CminsHS2R	Antisense	GTATAACGTCTGATCCAGTC	C. metapsilosis hot spot 2 sequencing
CpFKS1expF	Sense	ATCCAAGATCTTCCGGTGCCTCAA	Expression profiling
CpFKS1expR	Antisense	ATCAGCTGACCATGCTGGATATGG	Expression profiling
CpFKS2expF	Sense	AATGGGCAGAGGTTGAGAAGGTAG	Expression profiling
CpFKS2expR	Antisense	GGGTTCCAAGCAGGATATGGATCA	Expression profiling
CpFKS3expF	Sense	TCGTAGGTTCGAATCCTGCTGAGA	Expression profiling
CpFKS3expR	Antisense	ATGGTGAAGGCGCAACGGTGTAAA	Expression profiling
CaFKS1expF	Sense	TGATACTGGTAATCATAGACCAAAAA	Expression profiling
CaFKS1expR	Antisense	AACTCTGAATGGATTTGTAGAATAAGG	Expression profiling
CaFKS2expF	Sense	ACTTGCTAGCAGTCGCCAAT	Expression profiling
CaFKS2expR	Antisense	ACCACCATGAGCGGTTAGAC	Expression profiling
CaFKS3expF	Sense	ACCTCAATATTCAGCTTGGTGCCC	Expression profiling
CaFKS3expR	Antisense	GGACAACTCATTCGACTTGACCGT	Expression profiling
CgFKS1expF	Sense	CAATTGGCAGAACACCGATCCCAA	Expression profiling
CgFKS1expR	Antisense	AGTTGGGTTGTCCGTACTCATCGT	Expression profiling
CgFKS2expF	Sense	TACCAACCAGAAGACCAACAGAATGG	Expression profiling
CgFKS2expR	Antisense	TCACCACCGCTGATGTTTGGGT	Expression profiling
CgFKS3expF	Sense	GGGAGAGCACGTAAACGTAACTCAA	Expression profiling
CgFKS3expR	Antisense	TTTGCTGCTGTAAGGTTAGTGGCG	Expression profiling
But33	Sense	ATGATAGAGTTGAAAGTAGTTTGGTCAATA	Expression profiling (C. parapsilosis housekeeping gene)
But34	Antisense	ACTACTGCTGAAAGAGAAATTGTTAGAGAC	Expression profiling (C. parapsilosis housekeeping gene)
CaURA3F	Sense	CAACACTAAGACCTATAGTGAGAGAGC	Expression profiling (C. albicans housekeeping gene)
CaURA3R	Antisense	TGCACATAAATTGGTTTTCTTCA	Expression profiling (C. albicans housekeeping gene)
CgURA3F	Sense	CGAGAACACTGTTAAGCCATTG	Expression profiling (C. glabrata housekeeping gene)
CgURA3R	Antisense	CACCATGAGCGTTGGTGATA	Expression profiling (C. glabrata housekeeping gene)

^aSequences corresponding to URA3 flanking sequences in pRS416 are underlined.

^bCodon 647, incorporating N into the first position, is underlined.