TABLE 3.

Virulence of nfxB mutants

Strain	Rhamnolipid productiona	Production (%)b of:			T3SS		Colony size (%)f
		Elastasec	Phospholipase Cd	Pyocyaninc	Genotype	Cytotoxicity (%)e
PAO1	++	100	100	100	exoS	79 ± 5	100
EryR	+	60 ± 4	125 ± 16	57 ± 8	exoS	72 ± 16	35 ± 9
PA14	++	296 ± 28	156 ± 26	89 ± 12	exoU	79 ± 4	73 ± 11
KJ0707	+	222 ± 21	11 ± 4	36 ± 2	exoU	75 ± 3	32 ± 7
4.2	++	160 ± 13	87 ± 6	148 ± 22	exoS	39 ± 3	107 ± 21
KJ0705	++	31 ± 2	78 ± 10	33 ± 6	exoS	33 ± 5	23 ± 5
19.1	++	191 ± 16	45 ± 12	152 ± 36	exoS exoU	77 ± 5	210 ± 37
KJ0711	±	198 ± 7	55 ± 6	87 ± 7	exoS exoU	76 ± 2	49 ± 12
3308	+	180 ± 13	91 ± 3	64 ± 11	exoS	70 ± 8	65 ± 17
2439	+	15 ± 4	95 ± 18	5 ± 3	exoS	84 ± 8	34 ± 13
2126	++	167 ± 12	68 ± 6	74 ± 6	exoS	71 ± 23	25 ± 6
1956	++	291 ± 25	36 ± 10	98 ± 13	exoS	47 ± 10	24 ± 7

^aSemiquantitative estimation (from no production for an RhlR-negative mutant to the production level of PAO1 [++]) from triplicate samples. ±, standard deviation.

^bRelative to PAO1 (100%). Values in boldface correspond to significant reduction in production in nfxB mutants compared with wild-type parents.

^cValues are means of three independent experiments.

^dValues are means of four independent experiments.

^eExtracellular LDH activity at 3 h postinfection expressed as % of total lysis obtained with Triton X-100. Values are means of three experiments.

^fBacteria per colony relative to PAO1 (100%). Values are means of five determinations. Values in boldface correspond to significant reduction in colony size in nfxB mutants compared with wild-type parents.