TABLE 1.
Primers used in this study
| Primer | Nucleotide sequence (5′-3′)a | Position (bp) in blaCTX-M-3 sequence (DQ328958b) |
|---|---|---|
| A77V-F | TGATGGCCGTGGCCGCGGTGCTGAA | 229-254 |
| A77V-R | TTCAGCACCGCGGCCACGGCCATCA | 254-229 |
| N106S-F | CCTTGTTAACTATAGTCCGATT | 311-333 |
| N106S-R | CCGCAATCGGACTATAGTT | 337-318 |
| P167S-F | CGTACCGAGTCGACGTTAAACA | 498-520 |
| P167S-R | TGTTTAACGTCGACTCGGTACG | 520-498 |
| D240G-F | AAACCGGCAGCGGTGGCTAT | 709-728 |
| D240G-R | ATAGCCACCGCTGCCGGTTT | 729-709 |
| CTX-MEco-FW | GGAATTCGACTATTCATGTTGTTGTTATT | −47-26 upstream bla |
| CTX-MPst-RV | AACTGCAGTTCCGCTATTACAAACCGT | Including codon stop |
a
The EcoRI and PstI restriction sites are underlined in primers CTX-M-Eco and CTX-M-Pst, respectively. The italic letters in primer CTX-M-Pst represent the codon stop in blaCTX-M-3.
b
GenBank accession number.