TABLE 2.
Specificities of qPCR probes
| Target present | Probe used |
CTa
|
|
|---|---|---|---|
| Sybrb | HEXc | ||
| TEM-12 | TEM-12 | 6.92 | |
| TEM-12 (10−1) | TEM-12 | 9.93 | |
| TEM-12 (10−3) | TEM-12 | 17.9 | |
| TEM-12 | TEM-1 | No CT | |
| TEM-12 (10−1) | TEM-1 | No CT | |
| TEM-12 (10−3) | TEM-1 | No CT | |
| TEM-1 (10−2) | TEM-1 | 7.52 | |
| TEM-1 (10−4) | TEM-1 | 14.94 | |
| TEM-1 (10−2) | TEM-12 | No CT | |
| TEM-1 (10−4) | TEM-12 | No CT | |
| TEM-12, TEM-1 (10−2) | TEM-1, TEM-12 | 7.94 | 11.84 |
| TEM-12, TEM-1 (10−4) | TEM-1, TEM-12 | 15.26 | 19.84 |
| TEM-12, TEM-1 (10−2) | TEM-1 | 8.24 | |
| TEM-12, TEM-1 (10−2) | TEM-12 | 18.17 | |
| Water control | TEM-1 | No CT | |
| Water control | TEM-12 | No CT | |
a
CT (threshold cycle) refers to the first cycle at which the instrument can distinguish the amplification-generated fluorescence as being above the background signal. The CT value is inversely proportional to the initial target concentration.
b
TEM-1-specific probe was labeled with Sybr green.
c
TEM-12-specific probe was labeled with HEX (fluorescent label with excitation wavelength of 535 and emission wavelength of 555).