TABLE 3.
PCR linkage in S. enterica strains containing SGI1 or SGI2a
| Strain | SGI | Amplification of PCR product
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| thdF-int | S044-yidY | S026-intI1 | IS6100-S044 | S023-intI1 | IS6100-S024 | S026-S044 | S023-S024 | ||
| SRC49 | SGI1 | + | + | + | + | − | − | − | + |
| SRC19 | SGI2 | + | + | − | − | + | + | + | − |
| SRC234 | SGI2 | + | + | − | − | + | + | + | − |
| SRC235 | SGI2 | + | + | − | − | + | + | + | − |
| SRC239 | SGI2 | + | + | − | − | + | + | + | − |
a
Primer pairs linking thdF-int (U7-L12/LJ-R1), S044-yidY (104-RJ/104-D), S026-intI1 (S026-FW/int-RV), and IS6100-S044 (DB-T1/MDR-B) and their product sizes have been reported elsewhere (3, 9, 22). When no integron is present, S026-S044 (S026-FW/MDR-B) yields a product of 1.82 kb and S023-S024 [SGI19(F3)/S024-RV] yields a product of 2.92 kb. Primer pairs linking S023-intI1 [SGI19(F3)/int-RV] and IS6100-S024 (IS6100-F/S024-RV) yield products of 1.37 and 2.56 kb when the integron is in S023. Primer SGI19(F3) is from reference 22, and primer IS6100-F (5′-AAG GGA TTC GAA GTC ATG C-3′; matches positions 41322 to 41340 in IS6100 [GenBank accession no. AF261825]) is from this study.