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. Author manuscript; available in PMC: 2008 Jul 8.
Published in final edited form as: Dev Dyn. 2008 Apr;237(4):1060–1069. doi: 10.1002/dvdy.21485

Figure 1. RT-PCR and in situ analysis of the spatial and temporal expression pattern of zebrafish sip1a and sip1b.

Figure 1

(A) RT-PCR of zebrafish sip1a and sip1b with primers flaking Exon9 using mRNA isolated from wild type embryos at 0, 24, 48 and 72hpf. Each gene displayed two bands corresponding to two alternatively spliced mRNAs. (B–U) Wholemount in situ hybridized embryos hybridized with either a sip1a (B, C, F, G, J, K, N, O, R, S) or sip1b (D, E, H, I, L, M, P, Q, T, U) antisense probes at the indicated developmental stages. The first and third columns are lateral views; the second and fourth columns are dorsal views. Anterior is to the left. Red arrowhead indicate pre-otic neural crest (G), green arrowhead indicate post-otic neural crest (G), pink arrowheads indicate VII/VIII cranial ganglia (H, I, L, M), light blue arrowheads indicate V cranial ganglia (L, M), black arrowheads indicate pharyngeal arches (J, N), white arrow heads indicate gut mesendoderm (N).