Table 3.
Aggregation of ϕ29 and gp13-defective mutants with antibodies to tail components
| Expt. | Particlea | Antibody | Total Particles | DNA Filled (%) | Particles Aggregated (%) |
|---|---|---|---|---|---|
| 1 | WT (1241) | gp13 | 574 | 549 (96) | 552 (96) |
| 13- (342) | gp13 | 272 | 64 (24) | 53 (20) | |
| 2 | 13- (330) | gp13 | 299 | 201 (67) | 6 (2) |
| 13- (342) | gp13 | 300 | 243 (81) | 189 (63) | |
| 3 | 13- (330) | gp13 | 355 | 257 (72) | 5 (1) |
| 13- (330) | gp9 | 501 | 444 (89) | 322 (64) | |
| 13- (330) | p.i.b | 305 | 215 (71) | 9 (3) |
Aggregation of ϕ29 and gp13-defective mutants with specific immunoglobulins was determined by immuno-electron microscopy. Background aggregation is less than 5% as determined by pre-immune (p.i.) IgG incubation with 13(330) particles.
a
Numbers in parentheses indicate the phage mutant used.
b
p.i. indicates pre-immune immunoglobulins isolated from the same animal used to produce the anti-gp13 IgG.