Fig. 6.

Adiposity and HO-1 activity in bone marrow. A: Representative sections of bone marrow stained to reveal lipid droplets as described in Materials and Methods are shown for vehicle-treated lean mice (left panel), vehicle-treated obese mice (middle panel), and L-4F-treated obese mice (right panel) after 6 weeks of treatment. B: The percentage of bone marrow area with Oil Red O staining was calculated by measuring 10 fields for each sample at a magnification of ×20 as described in Materials and Methods. *P < 0.001 vehicle-treated obese mice versus vehicle-treated lean mice; ^#P < 0.05 vehicle-treated obese versus L-4F-treated obese mice. C: Bone marrow cells were harvested, and cell lysate was prepared using cell lysis buffer as described in Materials and Methods. HO activity was determined as described in Materials and Methods. *P < 0.014 vehicle-treated obese mice versus vehicle-treated lean mice; **P < 0.014 vehicle-treated obese mice versus L-4F-treated obese mice.