Fig. 6.

Effect of fatty acyl chain unsaturation on the kinetic disk stability. Thermal unfolding kinetics of apoC-I complexes with diunsaturated PLPC (light gray), monounsaturated palmitoyl-oleoyl PC (POPC) (dark gray triangles), and fully saturated DPPC (black, filled squares) and DSPC (black open circles) was analyzed in T-jumps. A: Time course of heat denaturation monitored by CD at 222 nm in a T-jump to 60°C; the unfolding of apoC-I complexes with DSPC (not shown) is extremely slow at 60°C. B: Arrhenius plots for the 1st kinetic phase of disk denaturation. The plots for apoC-I complexes with POPC and palmitoyl-linoleoyl PC (PLPC) (dark and light gray) significantly overlap, suggesting comparable stability of these complexes at 37°C. Solid lines show linear fits; double arrows indicate differences in rHDL stability at 37°C.