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Fig. 1.

Fig. 1.

HPLC profiles of a standard lutein (LUT) and zeaxanthin (ZEA) mixture (A), an extract from 7 week differentiated ARPE-19 cells after 20 h of incubation with 1 μM LUT (B), and an extract from 7 week differentiated ARPE-19 cells after 20 h of incubation with 1 μM ZEA (C). HPLC conditions were as follows: a YMC C30 column (250 × 4.6 mm, 3 μm), methanol-methyl-tert-butyl-ether (90:10, v/v) as mobile phase, and a flow rate at 0.9 ml/min (see Materials and Methods). A, B, and C, unidentified peaks; AU, absorbance units; PS, peak solvent.