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. 2008 Aug;49(8):1715–1724. doi: 10.1194/jlr.M700580-JLR200

Fig. 3.

Fig. 3.

Effects of the mode of delivery of carotenoids to ARPE-19 cells on the cellular uptake of β-C (b-C), LUT, and ZEA. A: β-C, LUT, or ZEA at 1 μM was solubilized in Tween 40 as described previously (32) and detailed in Materials and Methods. The resulting carotenoid-Tween suspension was applied to 7 week differentiated ARPE-19 cells. B: At time 0 of the experiment, 3 week differentiated Caco-2 cell monolayers on inserts were placed on top of 7 week differentiated ARPE-19 cells, and β-C, LUT, or ZEA at 10 μM in Tween 40 was applied at the apical side of Caco-2 cells in the presence of oleic acid and taurocholate to produce carotenoid-enriched chylomicrons (31). Carotenoids were thus delivered to ARPE-19 cells via lipoproteins produced by Caco-2 cells at a final concentration of 1 μM. After 18 h of exposure with one of the carotenoids (either solubilized in Tween 40 or carried by lipoproteins), carotenoid contents in cells and cell culture media were determined by HPLC. Data are means ± SD of two independent experiments.