Fig. 5.

Effects of antibodies against scavenger receptor class B type I (SR-BI; anti-SR-BI) and cluster determinant 36 (CD36; anti-CD36) on β-C (b-C) and ZEA uptake by ARPE-19 cells. At 7 weeks of differentiation, cells were preincubated with nonimmunized rabbit anti-IgG, anti-CD36, or anti-SR-BI at 8 μg protein/ml for 2 h, followed by incubation with β-C or ZEA in Tween 40 for 18 h. The absolute control experiment corresponded to cells incubated with the carotenoid alone (without pretreatment). The negative control (Neg. CTL) corresponded to the nonimmunized rabbit anti-IgG pretreatment. After incubation with carotenoids, cells were harvested and their carotenoid contents analyzed by HPLC. Data are expressed as percentage of the absolute control and presented as means ± SD of three independent experiments. ^a,b P < 0.001.