Fig. 1.

SDS-PAGE analysis of G-hLAL (human lysosomal acid lipase expressed in Nicotiana benthamiana using the GENEWARE® expression system) purification. Protein samples taken at each step of the purification procedure were separated by SDS-PAGE using a 10–20% Tris-glycine gel and stained with Coomassie Brilliant Blue stain. Lane 1, protein molecular mass marker, with corresponding molecular mass shown at left; lane 2, crude plant interstitial fluid extract; lane 3, proteins removed by centrifugation; lane 4, material loaded on a butyl Sepharose column; lane 5, pooled fractions eluted from butyl Sepharose; lane 6, material loaded onto an SP Sepharose column; lane 7, pooled fractions eluted from SP Sepharose; lane 8, dialyzed product before 0.2 μm filtration and vialing. The migration position of G-hLAL is indicated by the arrow.