Fig. 8.

Dose-dependent reduction of macrophages in tissues of G-hLAL-treated lal^−/− mice. Immunohistochemical staining of macrophages in the liver (A–D), spleen (E–H), and small intestine sections (I–L) from age-matched lal^−/− mice untreated (A, E, I), or G-hLAL-treated with 24 U (B, F, J), 48 U (C, G, K), and 72 U (D, H, L). The macrophages were dark-brown color by immunohistochemical staining with rabbit anti-mouse macrophage membrane antibody. A dose-dependent reduction of lipid-laden Kupffer cells, splenic macrophages, and intestinal macrophages of G-hLAL-treated lal^−/− mice (arrows) was evident. Regular Kupffer cells, which are not lipid laden, are indicated by arrowheads.