Figure 6. Tomosyn delays the immobilization of newcomer vesicles before fusion.

(a). Point-to-point velocity (ptpv) of newcomer (green) and resident (magenta) vesicles that fused in response to stimulation in control cells. The graph depicts the ptpv of vesicles during the first 4 s of their arrival near the membrane and then during the last 3 s before fusion. The ptpv of resident vesicles is shown for the first 4 s of acquisition and the last 3 s before fusion (magenta). (b). As in a, ptpv for resident and newcomer vesicles in tomosyn-overexpressing cells.