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. 1999 Dec 7;96(25):14360–14365. doi: 10.1073/pnas.96.25.14360

Figure 5.

Figure 5

The FA mutations in the polo-box do not significantly influence the expression level of PlkΔN. The wild-type 1788 cells bearing various YCplac22-GAL1-HA-EGFP-PlkΔN constructs were cultured under inducing conditions for 2 hr and then harvested. An equal amount (200 μg each) of total cellular lysates prepared from various transformants was loaded onto each lane. After the proteins were transferred onto a poly(vinylidene difluoride) membrane, proteins interacting with the anti-GFP Ab were detected by immunoblotting. EGFP, YCplac22-GAL1-HA-EGFP; EGFP-PlkΔN, YCplac22-GAL1-HA-EGFP-PlkΔN; EGFP-PlkΔN/FA, YCplac22-GAL1-HA-EGFP-PlkΔN/W414F/L427A. Although the expression level of the FA mutant induced for 10 hr becomes greater than that of wild type induced for 2 hr, it failed to yield distinctly localized signals (see text).