Figure 5.

(Upper) Restriction map of TYS1 and flanking region. Numbers 1, 2, and 3 are reading frames from one direction, and −1, −2, and −3 are reading frames from the opposite direction. The half bars represent ATG codons, and the full bars represent stop codons. A segment of UBR1, which codes for ubiquitin-protein ligase, is present in the DNA fragment. TYS1 encodes tyrosyl-tRNA synthetase. TFG1 encodes the large subunit of transcription factor TFIIF. HGH1 encodes HMG1/2 homologue. BUB1 encodes a serine/threonine protein kinase. Abbreviations for restriction enzymes are as follows: B, BamHI; S, SacI; Sa, SalI. (Lower) Mapping of the TYS1 gene. Complete rescue of the growth of tys1-1 at 37°C is shown by + and nonrescue by − for each fragment.