Figure 3.

Hox expression in MEFs. (a) MEF lines prepared from Mll^+/+ and Mll^−/− embryos were examined for their expression pattern of Hoxa and c genes by a semiquantitative RNA RT-PCR analysis. Mll (AT-hooks) region is present in the disrupted (−/−) as well as wild-type (+/+) alleles whereas Mll (exon 3) is deleted, but β-galactosidase is inserted in the (−/−) alleles. RT signifies the presence (+) or absence (−) of reverse transcriptase. (b) Hoxa and c expression in Bmi-1^+/+ versus Bmi-1^−/− MEFs.