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Bmi-1 and MLL expression plasmids produce proteins with discrete subnuclear colocalization. (A–C) Transiently transfected 293 cells. (D–F) MEF stable transfectants were stained for HA-tagged BMI-1 (A, C, D, and F) and FLAG-tagged MLL (B, C, E, and F). Laser confocal microscopy demonstrates discrete subnuclear localization of both proteins with significant colocalization when the images are superimposed (C and F, yellow fluorescence).
