Table 1.
Expression of the Rfz-2/β2AR chimera in Chinese hamster ovary (CHO-K) cells deficient in β-ARs and in F9 cells yields increased expression of β-AR binding, as measured by the binding of the radiolabeled, high-affinity β-adrenergic antagonist [125I]CYP
Cell line | Vector | [125I]CYP binding, fmol/105 cells |
---|---|---|
CHO-K | EV | 0.6 ± 0.2 |
Rfz-2/β2AR chimera | 18.0 ± 2.5 | |
F9 | EV | 10.3 ± 0.9 |
Rfz-2/β2AR chimera | 20.6 ± 4.4 |
Data are expressed as the mean values (±SEM) of at least four different clones and two independent experiments. EV, empty vector control.