Diagram of the Adh gene of rice. Boxes represent exons numbered 1–10 from the 5′ to 3′ ends; lines between exons represent introns. Arrows indicate locations and directions of the PCR primers: AdhF1, 5′-CACACCGACGTCTACTTCTG-3′; AdhF2, 5′-AGAGTGTTGGAGAGGGTGTGAC-3′; AdhR1, 5′-ATCATGGCGTTGATGTTGCC-3′; Adh1bR, 5′-TCAGCAAGTACCTAAATTATC-3′; Adh1R4, 5′-TTCWGTGCAACCAAATTTCC-3′; Adh2RR, 5′-CCACCGTTGGTCATCTCAAT-3′; and Adh2R4, 5′-GTCAGTGCAGCCAAACTTCT-3′. Adh1bR and Adh1R4 are Adh1 gene-specific primers, and Adh2RR and Adh2R4 are Adh2 gene-specific primers. Information regarding internal sequencing primers is available from the authors.